Characteristics of the Epithelial-Mesenchymal Transition in Primary and Paired Metastatic Canine Mammary Carcinomas.

ABSTRACT: The epithelial-mesenchymal transition (EMT) is a dynamic process linked to metastasis in many tumor types, including mammary tumors. In this study, we evaluated E-cadherin and vimentin immunolocalization in primary canine mammary carcinomas (20 cases) and their respective metastases, as well as their relationship with the core regulators SNAIL/SLUG. To assess the number of cells undergoing the process of EMT, we quantitated double-positive (E-cadherin⁺/vimentin⁺) cells using immunofluorescence, via cell counting and image analysis. In addition, SNAIL/SLUG expression was evaluated by established immunohistochemical methods. Primary tumors had significantly more E-cadherin⁺/vimentin⁺ co-expression than their paired respective lymph node or distant

SUBMITTER: Raposo-Ferreira TMM 

PROVIDER: S-EPMC7993492 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature