Parameters and determinants of responses to selection in antibody libraries.

ABSTRACT: The sequences of antibodies from a given repertoire are highly diverse at few sites located on the surface of a genome-encoded larger scaffold. The scaffold is often considered to play a lesser role than highly diverse, non-genome-encoded sites in controlling binding affinity and specificity. To gauge the impact of the scaffold, we carried out quantitative phage display experiments where we compare the response to selection for binding to four different targets of three different antibody libraries based on distinct scaffolds but harboring the same diversity at randomized sites. We first show that the response to selection of an antibody library may be captured by two measurable parameters. Second, we provide evidence that one of these parameters is determined by the degree of affinity mat

SUBMITTER: Schulz S 

PROVIDER: S-EPMC7993935 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature