Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants.

ABSTRACT: Determining the molecular properties of neurons is essential to understand their development, function and evolution. Using Targeted DamID (TaDa), we characterize RNA polymerase II occupancy and chromatin accessibility in selected Ionotropic receptor (Ir)-expressing olfactory sensory neurons in Drosophila. Although individual populations represent a minute fraction of cells, TaDa is sufficiently sensitive and specific to identify the expected receptor genes. Unique Ir expression is not consistently associated with differences in chromatin accessibility, but rather to distinct transcription factor profiles. Genes that are heterogeneously expressed across populations are enriched for neurodevelopmental factors, and we identify functions for the POU-domain protein

SUBMITTER: Arguello JR 

PROVIDER: S-EPMC7993999 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature