Comparison of real-time and droplet digital PCR to detect and quantify SARS-CoV-2 RNA in plasma.

ABSTRACT:

Background

The presence of SARS-CoV-2 RNA in plasma has been linked to disease severity and mortality. We compared RT-qPCR to droplet digital PCR (ddPCR) to detect SARS-CoV-2 RNA in plasma from COVID-19 patients (mild, moderate, and critical disease).

Methods

The presence/concentration of SARS-CoV-2 RNA in plasma was compared in three groups of COVID-19 patients (30 outpatients, 30 ward patients and 30 ICU patients) using both RT-qPCR and ddPCR. Plasma was obtained in the first 24h following admission, and RNA was extracted using eMAG. ddPCR was performed using Bio-Rad SARS-CoV-2 detection kit, and RT-qPCR was performed using GeneFinder™ COVID-19 Plus RealAmp Kit. Statistical analysis was performed using Statistical Package for the Social Science.

Results

SARS-CoV-2 R

SUBMITTER: Tedim AP 

PROVIDER: S-EPMC7995030 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature