Large scale production and characterization of SARS-CoV-2 whole antigen for serological test development.

ABSTRACT:

Background

The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has generated a pandemic with alarming rates of fatality worldwide. This situation has had a major impact on clinical laboratories that have attempted to answer the urgent need for diagnostic tools, since the identification of coronavirus disease 2019 (COVID-19). Development of a reliable serological diagnostic immunoassay, with high levels of sensitivity and specificity to detect SARS-CoV-2 antibodies with improved differential diagnosis from other circulating viruses, is mandatory.

Methods

An enzyme-linked immunosorbent assay (ELISA) using whole inactivated virus cultured in vitro, was developed to detect viral antigens. WB and ELISA investigations were carried out with sera of conv

SUBMITTER: Cerutti H 

PROVIDER: S-EPMC7995104 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature