Fibrinogen heterogeneity in horses.

ABSTRACT:

Background

Fibrinogen heterogeneity has been observed in humans and can influence fibrinogen measurements when using the modified Clauss assay. We hypothesized that fibrinogen heterogeneity also exists in horses.

Objectives

To determine whether fibrinogen heterogeneity exists in horses.

Animals

Five clinically healthy horses from the university equine teaching herd.

Methods

Presumed fibrinogen was purified from pooled citrated plasma and electrophoresis performed. The purified protein was subjected to Western blotting using sheep antiserum against human fibrinogen, and liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results

Gel electrophoresis of nonreduced equine purified protein yielded 2 protein bands (approximately 377 and 318 kDa) that co

SUBMITTER: Russell EB 

PROVIDER: S-EPMC7995376 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature