Sphingosine-1-phosphate as a key player of insulin secretion induced by high-density lipoprotein treatment.

ABSTRACT: Beta cell failure is one of the most important features of type 2 diabetes mellitus (T2DM). High-density lipoprotein (HDL) has been proposed to improve β-cell function. However, the mechanisms involved in this process are still poorly understood. The aim of this study was to investigate the contribution of sphingosine-1-phosphate (S1P) in the impact of HDL treatment on insulin secretion by pancreatic β-cells and to determine its mechanisms. Primary cultures of β-cells isolated from rat were treated with or without HDL in the presence or absence of S1P pathway inhibitors and insulin secretion response was analyzed. The S1P content of HDL (HDL-S1P) isolated from T2DM patients was analyzed and correlated to the HDL-induced insulin secretion. The expression of genes involved in the biosynthesi

SUBMITTER: Brulhart-Meynet MC 

PROVIDER: S-EPMC7995544 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature