Research Techniques Made Simple: Use of Imaging Mass Cytometry for Dermatological Research and Clinical Applications.

ABSTRACT: Traditional immunohistochemistry (IHC) is inherently limited by its ability to analyze only several markers within a histological tissue section at a given time, which hinders in-depth characterization and phenotyping of tissues. Imaging mass cytometry (IMC), which combines IHC using metal-labeled antibodies with laser ablation and detection using mass cytometry by time-of-flight, overcomes this limitation with the capability to simultaneously analyze up to 40 protein markers to generate high-dimensional images from a single tissue section. IMC analysis preserves tissue architecture and spatial cellular relationships that would otherwise be lost or significantly altered in applications requiring tissue dissociation, such as flow cytometry or single-cell RNA sequencing. Resulting high-dimen

SUBMITTER: Veenstra J 

PROVIDER: S-EPMC7995633 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature