Isobaric Quantitative Protein Interaction Reporter Technology for Comparative Interactome Studies.

ABSTRACT: Chemical cross-linking with mass spectrometry (XL-MS) has emerged as a useful tool for the large-scale study of protein structures and interactions from complex biological samples including intact cells and tissues. Quantitative XL-MS (qXL-MS) provides unique information on protein conformational and interaction changes resulting from perturbations such as drug treatment and disease state. Previous qXL-MS studies relied on the incorporation of stable isotopes into the cross-linker (primarily deuterium) or metabolic labeling with SILAC. Here, we introduce isobaric quantitative protein interaction reporter (iqPIR) technology which utilizes stable isotopes selectively incorporated into the cross-linker design, allowing for isobaric cross-linked peptide pairs originating from different samples

SUBMITTER: Chavez JD 

PROVIDER: S-EPMC7995634 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature