Respiratory and systemic impacts following MWCNT inhalation in B6C3F1/N mice.

ABSTRACT:

Background

A very pure multi-walled carbon nanotube (MWCNT) that was shown to have very low toxicity in vitro, was evaluated for lung and systemic effects and distribution following inhalation exposure.

Methods

B6C3F1/N mice were exposed to varying doses (0, 0.06, 0.2, and 0.6 mg/m³) of the (99.1% carbon) MWCNT by inhalation for 30 days (excluding weekends). Ten days following the last exposure, the lungs and spleen were harvested and processed for histology and immune cell population assessment. In addition, lung lavage cells and fluid were analyzed. Stimulated Raman scattering (SRS) was used to identify particles in the lungs, spleen, kidneys, liver, mediastinal and brachial lymph nodes, and olfactory bulb. Splenic tissue sections were stained with hematoxylin a

SUBMITTER: Migliaccio CT 

PROVIDER: S-EPMC7995731 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature