Impact of taurine on red blood cell metabolism and implications for blood storage.

ABSTRACT:

Background

Taurine is an antioxidant that is abundant in some common energy drinks. Here we hypothesized that the antioxidant activity of taurine in red blood cells (RBCs) could be leveraged to counteract storage-induced oxidant stress.

Study design and methods

Metabolomics analyses were performed on plasma and RBCs from healthy volunteers (n = 4) at baseline and after consumption of a whole can of a common, taurine-rich (1000 mg/serving) energy drink. Reductionistic studies were also performed by incubating human RBCs with taurine ex vivo (unlabeled or ¹³ C¹⁵ N-labeled) at increasing doses (0, 100, 500, and 1000 μmol/L) at 37°C for up to 16 hours, with and without oxidant stress challenge with hydrogen peroxide (0.1% or 0.5%). Finally, we stored human

SUBMITTER: Bertolone L 

PROVIDER: S-EPMC7995806 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature