An organoid model to assay the role of CFTR in the human epididymis epithelium.

ABSTRACT: Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used human epididymis epithelial cell (HEE) organoids and polarized HEE cell cultures to assay the CF transmembrane conductance regulator (CFTR) in the human epididymis. 3D HEE organoids and polarized 2D HEE cell cultures on membrane inserts were established from human caput epididymis. Single-cell RNA sequencing (scRNA-seq) was performed to map cell type-specific gene expression in the organoids. Using forskolin (FSK) to activate CFTR and inhibitor CFTRinh₁₇₂ to block its activity, we assess

SUBMITTER: Leir SH 

PROVIDER: S-EPMC7996043 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature