Harnessing Colon Chip Technology to Identify Commensal Bacteria That Promote Host Tolerance to Infection.

ABSTRACT: Commensal bacteria within the gut microbiome contribute to development of host tolerance to infection, however, identifying specific microbes responsible for this response is difficult. Here we describe methods for developing microfluidic organ-on-a-chip models of small and large intestine lined with epithelial cells isolated from duodenal, jejunal, ileal, or colon organoids derived from wild type or transgenic mice. To focus on host-microbiome interactions, we carried out studies with the mouse Colon Chip and demonstrated that it can support co-culture with living gut microbiome and enable assessment of effects on epithelial adhesion, tight junctions, barrier function, mucus production, and cytokine release. Moreover, infection of the Colon Chips with the pathogenic bacterium, Salmonel

SUBMITTER: Gazzaniga FS 

PROVIDER: S-EPMC7996096 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature