Dataset Information

Panax ginseng-Derived Extracellular Vesicles Facilitate Anti-Senescence Effects in Human Skin Cells: An Eco-Friendly and Sustainable Way to Use Ginseng Substances.

ABSTRACT: Ginseng is a traditional herbal medicine in eastern Asian countries. Most active constituents in ginseng are prepared via fermentation or organic acid pretreatment. Extracellular vesicles (EVs) are released by most organisms from prokaryotes to eukaryotes and play central roles in intra- and inter-species communications. Plants produce EVs upon exposure to microbes; however, their direct functions and utility for human health are barely known, except for being proposed as delivery vehicles. In this study, we isolated EVs from ginseng roots (GrEVs) or the culture supernatants of ginseng cells (GcEVs) derived from Panax ginseng C.A. Meyer and investigated their biological effects on human skin cells. GrEV or GcEV treatments improved the replicative senescent or senescence-associated pigmented phenotypes of human dermal fibroblasts or ultraviolet B radiation-treated human melanocytes, respectively, by downregulating senescence-associated molecules and/or melanogenesis-related proteins. Based on comprehensive lipidomic analysis using liquid chromatography mass spectrometry, the lipidomic profile of GrEVs differed from that of the parental root extracts, showing significant increases in 70 of 188 identified lipid species and prominent increases in diacylglycerols, some phospholipids (phosphatidylcholine, phosphatidylethanolamine, lysophosphatidylcholine), and sphingomyelin, revealing their unique vesicular properties. Therefore, our results imply that GEVs represent a novel type of bioactive and sustainable nanomaterials that can be applied to human tissues for improving tissue conditions and targeted delivery of active constituents.

SUBMITTER: Cho EG

PROVIDER: S-EPMC7996359 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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<i>Panax ginseng</i>-Derived Extracellular Vesicles Facilitate Anti-Senescence Effects in Human Skin Cells: An Eco-Friendly and Sustainable Way to Use Ginseng Substances.

Cho Eun-Gyung EG Choi Suh-Yeon SY Kim Hyoseon H Choi Eun-Jeong EJ Lee Eun-Jeong EJ Park Phil-Jun PJ Ko Jaeyoung J Kim Kwang Pyo KP Baek Heung Soo HS

Cells 20210224 3

Ginseng is a traditional herbal medicine in eastern Asian countries. Most active constituents in ginseng are prepared via fermentation or organic acid pretreatment. Extracellular vesicles (EVs) are released by most organisms from prokaryotes to eukaryotes and play central roles in intra- and inter-species communications. Plants produce EVs upon exposure to microbes; however, their direct functions and utility for human health are barely known, except for being proposed as delivery vehicles. In t ...[more]

PMID: 33668388

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Project description:ObjectivesThe current study aimed to investigate how regulatory frameworks and policies are used to support the use of sustainable medicines within the pharmaceutical sector.MethodsThe Scopus database was searched to retrieve papers. Advanced search tool of the Scopus online database was used focused on the papers that have the search query included in their titles. Data analysis incorporated bibliometric indicators like publication counts and trends, visualized through VOSviewer software version 1.6.20.Key findingsA total of 43 publications on Sustainable Medicines Use were found between 2000 and 2024. Leading countries in publication output on sustainable medicines use were United Kingdom, United States, India, Italy, Portugal, and Switzerland, indicating their collaborative relationships and publication volumes. A total of 92 institutions have been involved in research on Sustainable Medicines Use. Key institutions such as the Faculty of Engineering and the Laboratory for Process and Environmental Engineering, Lowell Center for Sustainable Production, Greiner Environmental Inc., and the University of Florence are prominently featured, indicating their significant contributions to research in this area. Key journals such as the "Journal of Cleaner Production," "Business Strategy and the Environment," "Chemical Engineering Transactions," "Benchmarking," and "Lecture Notes in Mechanical Engineering" are prominently featured. The retrieved articles have been cited an average count of 22.26. The overlay visualization created using VOSviewer suggest a shift towards exploring new drug categories, innovative approaches, and the commercial aspects of sustainability. Future research directions are likely to delve deeper into innovative methods and sustainable chemical practices (green chemistry), reflecting an emphasis on developing greener processes and products.ConclusionThis study offers a thorough analysis of the legislative and governmental strategies promoting the use of sustainable medicine. It offers important insights for promoting sustainability in the pharmaceutical industry by pointing out gaps, defining useful frameworks, and suggesting doable solutions. Achieving sustainable pharmaceutical practices that support worldwide environmental and public health objectives requires sustained research, policy development, and international cooperation. The area needs to keep developing and implementing sustainable methods like green chemistry to decrease environmental harm and improve sustainability. Furthermore, collaborations among academia, industry, and international organizations are essential to progress and interchange effective strategies.

Project description:Malva parviflora L. is an edible and medicinal herb containing mucilaginous cells in its leaves. Mucilage obtained from M. parviflora leaves (MLM) was extracted in distilled water (1:10 w/v) at 70 °C followed by precipitation with alcohol. Preliminary phytochemical tests were performed to assess the purity of the extracted mucilage. Results showed that the yield of mucilage was 7.50%, and it was free from starch, alkaloids, glycosides, saponins, steroids, lipids and heavy metals. MLM had 16.19% carbohydrates, 13.55% proteins and 4.76% amino acids, which indicate its high nutritional value. Physicochemical investigations showed that MLM is neutral and water-soluble, having 5.84% moisture content, 15.60% ash content, 12.33 swelling index, 2.57 g/g water-holding capacity and 2.03 g/g oil-binding capacity. The functional properties, including emulsion capacity, emulsion stability, foaming capacity and stability increased with increased concentrations. Micromeritic properties, such as bulk density, tapped density, Carr's index, Hausner ratio, and angle of repose, were found to be 0.69 g/cm3, 0.84 g/cm3, 17.86%, 1.22 and 28.5, respectively. Scanning electron microscopy (SEM) showed that MLM is an amorphous powder possessing particles of varying size and shape; meanwhile, rheological studies revealed the pseudoplastic behavior of MLM. The thermal transition process of MLM revealed by a differential scanning calorimetry (DSC) thermogram, occurring at a reasonable enthalpy change (∆H), reflects its good thermal stability. The presence of functional groups characteristic of polysaccharides was ascertained by the infrared (IR) and gas chromatography/mass spectrometry (GC/MS) analyses. GC revealed the presence of five neutral monosaccharides; namely, galactose, rhamnose, arabinose, glucose and mannose, showing 51.09, 10.24, 8.90, 1.80 and 0.90 mg/g of MLM, respectively. Meanwhile, galacturonic acid is the only detected acidic monosaccharide, forming 15.06 mg/g of MLM. It showed noticeable antioxidant activity against the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical with an IC50 value of 154.27 µg/mL. It also prevented oxidative damage to DNA caused by the Fenton reagent, as visualized in gel documentation system. The sun protection factor was found to be 10.93 ± 0.15 at 400 µg/mL. Thus, MLM can be used in food, cosmetic and pharmaceutical industry and as a therapeutic agent due to its unique properties.

Dataset Information

Panax ginseng-Derived Extracellular Vesicles Facilitate Anti-Senescence Effects in Human Skin Cells: An Eco-Friendly and Sustainable Way to Use Ginseng Substances.

Publications

<i>Panax ginseng</i>-Derived Extracellular Vesicles Facilitate Anti-Senescence Effects in Human Skin Cells: An Eco-Friendly and Sustainable Way to Use Ginseng Substances.

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