Screening Methods for Isolation of Biocontrol Epiphytic Yeasts against Penicillium digitatum in Lemons.

ABSTRACT: Worldwide, the green rot caused by Penicillium digitatum is one of the most aggressive postharvest diseases of lemons. Searching for sustainable alternatives to chemical fungicides, epiphytic yeasts as potential biocontrol agents were isolated from citrus fruits using a tailor-made selective medium. For disclosing their antagonistic potential against P. digitatum, obtained isolates were subjected to direct screening methods, both in vitro and in vivo. In the course of the primary in vitro screening that comprised dual culture assays, 43 yeast strains displaying antagonistic activities against the pathogen were selected. Subsequently, such strains were subjected to an in vivo screening that consisted of a microscale test, allowing the selection of six yeast strains for further

SUBMITTER: Pereyra MM 

PROVIDER: S-EPMC7996618 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature