A New Duplex PCR-Assay for the Detection and Identification of Paracoccidioides Species.

ABSTRACT: Paracoccidioidomycosis (PCM) is a life-threatening systemic fungal infection caused by members of the Paracoccidioides brasiliensis complex and P. lutzii. Routine diagnoses of PCM down to the species level using classical mycological approaches are unspecific due to overlapping phenotypes. There is an urgent need for specific, sensitive, and cost-effective molecular tools to diagnose PCM. Variation among the exon-2 of the gp43 gene was exploited to design species-specific primer pairs to discriminate between members of the P. brasiliensis complex and P. lutzii in a duplex PCR assay. Primer-BLAST searches revealed highly species-specific primers, and no significant region of homology was found against DNA databases except for Paracoccidioides species. Prim

SUBMITTER: Pinheiro BG 

PROVIDER: S-EPMC7996757 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature