A New Generation of Functional Tagged Proteins for HIV Fluorescence Imaging.

ABSTRACT: During the last decade, there was a marked increase in the development of tools and techniques to study the molecular mechanisms of the HIV replication cycle by using fluorescence microscopy. Researchers often apply the fusion of tags and fluorophores to viral proteins, surrogate proteins, or dyes to follow individual virus particles while they progress throughout infection. The inclusion of such fusion motifs or surrogates frequently disrupts viral infectivity or results in a change of the wild-type phenotype. Here, we detail the construction and functional characterization of two new constructs where we fused fluorescent proteins to the N-terminus of HIV-1 Integrase. In the first, IN is recruited into assembling particles via a codon optimized Gag to complement other viral constructs, wh

SUBMITTER: Mamede JI 

PROVIDER: S-EPMC7997544 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature