Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection.

ABSTRACT: COVID-19 is currently a highly pressing health threat and therapeutic strategies to mitigate the infection impact are urgently needed. Characterization of the SARS-CoV-2 interactome in infected cells may represent a powerful tool to identify cellular proteins hijacked by viruses for their life cycle and develop host-oriented antiviral therapeutics. Here we report the proteomic characterization of host proteins interacting with SARS-CoV-2 Nucleoprotein in infected Vero E6 cells. We identified 24 high-confidence proteins mainly playing a role in RNA metabolism and translation, including RNA helicases and scaffold proteins involved in the formation of stress granules, cytoplasmic aggregates of messenger ribonucleoproteins that accumulate as a result of stress-induced translation arrest. Analy

SUBMITTER: Ciccosanti F 

PROVIDER: S-EPMC7997689 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature