Dataset Information

Dolichos kongkandae sp. nov. and lectotypification of D. fragrans (Leguminosae, Papilionoideae) from Asia.

ABSTRACT: Dolichos kongkandae is described as a new species from Asia and includes a line drawing, photographs and information on its distribution and ecology. The morphological differences between D. kongkandae and the morphologically similar D. tenuicaulis are highlighted and clarified. Additionally, a lectotype for D. fragrans is designated.

SUBMITTER: Meeboonya R

PROVIDER: S-EPMC7997857 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Dolichos kongkandae sp. nov. and lectotypification of D. fragrans (Leguminosae, Papilionoideae) from Asia.

Meeboonya Rumrada R Ngernsaengsaruay Chatchai C Balslev Henrik H

PhytoKeys 20210319

Dolichos kongkandae is described as a new species from Asia and includes a line drawing, photographs and information on its distribution and ecology. The morphological differences between D. kongkandae and the morphologically similar D. tenuicaulis are highlighted and clarified. Additionally, a lectotype for D. fragrans is designated. ...[more]

PMID: 33814952

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Project description:Background and aimsSpecies of the genus Burkholderia, from the Betaproteobacteria, have been isolated from legume nodules, but so far they have only been shown to form symbioses with species of Mimosa, sub-family Mimosoideae. This work investigates whether Burkholderia tuberum strains STM678 (isolated from Aspalathus carnosa) and DUS833 (from Aspalathus callosa) can nodulate species of the South African endemic papilionoid genera Cyclopia (tribe Podalyrieae) and Aspalathus (Crotalarieae) as well as the promiscuous legume Macroptilium atropurpureum (Phaseoleae).MethodsBacterial strains and the phylogeny of their symbiosis-related (nod) genes were examined via 16S rRNA gene sequencing. Seedlings were grown in liquid culture and inoculated with one of the two strains of B. tuberum or with Sinorhizobium strain NGR 234 (from Lablab purpureus), Mesorhizobium strain DUS835 (from Aspalathus linearis) or Methylobacterium nodulans (from Crotalaria podocarpa). Some nodules, inoculated with green fluorescence protein (GFP)-tagged strains, were examined by light and electron microscopy coupled with immunogold labelling with a Burkholderia-specific antibody. The presence of active nitrogenase was checked by immunolabelling of nitrogenase and by the acetylene reduction assay. B. tuberum STM678 was also tested on a wide range of legumes from all three sub-families.Key resultsNodules were not formed on any of the Aspalathus spp. Only B. tuberum nodulated Cyclopia falcata, C. galioides, C. genistoides, C. intermedia and C. pubescens. It also effectively nodulated M. atropurpureum but no other species tested. GFP-expressing inoculant strains were located inside infected cells of C. genistoides, and bacteroids in both Cyclopia spp. and M. atropurpureum were immunogold-labelled with antibodies against Burkholderia and nitrogenase. Nitrogenase activity was also shown using the acetylene reduction assay. This is the first demonstration that a beta-rhizobial strain can effectively nodulate papilioinoid legumes.ConclusionsPapilionoid legumes from widely different tribes can be nodulated by beta-rhizobia, forming both indeterminate (Cyclopia) and determinate (Macroptilium) nodules.

Dataset Information

Dolichos kongkandae sp. nov. and lectotypification of D. fragrans (Leguminosae, Papilionoideae) from Asia.

Publications

<i>Dolichos kongkandae</i> sp. nov. and lectotypification of <i>D. fragrans</i> (Leguminosae, Papilionoideae) from Asia.

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