Extracellular vesicles are associated with C-reactive protein in sepsis.

ABSTRACT: There is increasing evidence that C-reactive protein (CRP) can mediate inflammatory reactions following the transformation of functionally inert pentameric CRP (pCRP) into its structural isoform pCRP* and into monomeric CRP (mCRP). This conversion can occur on the membranes of apoptotic or activated cells or on extracellular vesicles (EVs) shed from the cell surface. Here, we characterized the association of CRP with EVs in plasma from sepsis patients using flow cytometry, and found highly elevated levels of total EV counts and CRP⁺ EVs as compared to healthy individuals. We further assessed the ability of PentraSorb CRP, an extracorporeal device for the adsorption of CRP, to deplete free CRP and CRP⁺ EVs. Treatment of septic plasma with the adsorbent in vitro resulte

SUBMITTER: Fendl B 

PROVIDER: S-EPMC7997920 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature