Single-cell analyses of Crohn's disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions.

ABSTRACT: Crohn's disease (CD) is a chronic transmural inflammation of intestinal segments caused by dysregulated interaction between microbiome and gut immune system. Here, we profile, via multiple single-cell technologies, T cells purified from the intestinal epithelium and lamina propria (LP) from terminal ileum resections of adult severe CD cases. We find that intraepithelial lymphocytes (IEL) contain several unique T cell subsets, including NKp30⁺γδT cells expressing RORγt and producing IL-26 upon NKp30 engagement. Further analyses comparing tissues from non-inflamed and inflamed regions of patients with CD versus healthy controls show increased activated T_H17 but decreased CD8⁺T, γδT, T_FH and Treg cells in inflamed tissues. Similar analyses of LP fin

SUBMITTER: Jaeger N 

PROVIDER: S-EPMC7997960 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature