Effect of Electrolyte Concentration on Cell Sensing by Measuring Ionic Current Waveform through Micropores.

ABSTRACT: Immunostaining has been widely used in cancer prognosis for the quantitative detection of cancer cells present in the bloodstream. However, conventional detection methods based on the target membrane protein expression exhibit the risk of missing cancer cells owing to variable protein expressions. In this study, the resistive pulse method (RPM) was employed to discriminate between cultured cancer cells (NCI-H1650) and T lymphoblastoid leukemia cells (CCRF-CEM) by measuring the ionic current response of cells flowing through a micro-space. The height and shape of a pulse signal were used for the simultaneous measurement of size, deformability, and surface charge of individual cells. An accurate discrimination of cancer cells could not be obtained using 1.0 × phosphate-buffered saline (PBS)

SUBMITTER: Yokota K 

PROVIDER: S-EPMC7998150 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature