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Bioactivity and Control Efficacy of the Novel Antibiotic Tetramycin against Various Kiwifruit Diseases.

ABSTRACT: Tetramycin, a novel polyene agriculture antibiotic, has excellent antimicrobial activity against many plant pathogens. In this study, the antimicrobial activities of tetramycin and conventional antibiotics on eight common pathogens and their field control efficacies against four serious diseases in kiwifruit were investigated. The results show that 0.3% tetramycin aqueous solutions (AS) exhibited the superior antibacterial and antifungal activity against Pseudomonas syringae pv. actinidiae, Pseudomonas fulva, Agrobacterium tumefaciens, Botryosphaeriadothidea, Phomopsis sp., Alternaria tenuissima, Armillariella mellea and Phytophthora cactorum of kiwifruit pathogens with EC50 values of 1.21, 1.24, 0.72, 0.14, 0.09, 0.16, 0.06 and 0.17 mg kg-1, respectively. These EC50 values of tetramycin were much higher than those of conventional kasugamycin, zhongshengmycin or polyoxin. Meanwhile, 0.3% tetramycin AS possessed the good field control efficacies for canker, soft rot, blossom blight and brown spot disease of kiwifruit with 74.45, 83.55, 84.74 and 89.62%. Moreover, 0.3% tetramycin AS application notably increased fruit resistance substances contents, activated fruit superoxide dismutase and polyphenoloxidase activities, as well as remarkably enhanced fruit growth, improved fruit quality and storability. This study highlights that tetramycin can be used as a preferred alternative to conventional antibiotics in kiwifruit production.

SUBMITTER: Wang Q

PROVIDER: S-EPMC7998335 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Bioactivity and Control Efficacy of the Novel Antibiotic Tetramycin against Various Kiwifruit Diseases.

Wang Qiuping Q Zhang Cheng C Long Youhua Y Wu Xiaomao X Su Yue Y Lei Yang Y Ai Qiang Q

Antibiotics (Basel, Switzerland) 20210310 3

Tetramycin, a novel polyene agriculture antibiotic, has excellent antimicrobial activity against many plant pathogens. In this study, the antimicrobial activities of tetramycin and conventional antibiotics on eight common pathogens and their field control efficacies against four serious diseases in kiwifruit were investigated. The results show that 0.3% tetramycin aqueous solutions (AS) exhibited the superior antibacterial and antifungal activity against <i>Pseudomonas syringae</i> pv. <i>actini ...[more]

PMID: 33802124

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Project description:BackgroundAntibiotic resistance represents a serious global health threat to public health, so infections such as pneumonia and urinary tract infection (UTI) are becoming harder to treat. Therefore, it is necessary to develop an action plan to restrain the problem of antibiotic resistance. One approach in UTI control could be the use of lactobacilli because these indigenous inhabitants in human intestine have been found to play an important role in protecting the host from various infections.ObjectivesWe sought to check the efficacy of locally isolated Lactobacillus species to eradicate antibiotic-resistant pathogenic bacteria causing UTI.Materials and methodsLactic acid bacteria isolated from spoiled fruits and vegetables and grown in MRS medium were screened against multi-drug-resistant Candida albicans, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus fecalis.ResultsFifty-four lactic acid bacteria were isolated from spoiled fruits and vegetables, of which 11 Gram-positive and catalase-negative Lactobacillus isolates were identified by carbohydrate assimilation profiles as Lactobacillus acidophilus, L. paracasei, L. delbrueckii, L. casei, L. helveticus, L. brevis, L. salivarius, L. fermentum, L. rhamnosus, L. animalis, and L. plantarum. The latter organism had the highest abundance of all the samples, so its isolates were also verified through 16S rRNA gene sequencing. The isolated Lactobacilli were screened against multi-drug-resistant uropathogens, viz. C. albicans, P. aeruginosa, K. pneumoniae, E. fecalis, and E. coli. The growth inhibition zone (GIZ) was over 10 mm against all the uropathogenic test organisms, where L. fermentum and L. plantarum strains demonstrated remarkable inhibitory activities against E. coli and E. faecalis, with a GIZ up to 28 mm. The susceptibility test to 16 antibiotics showed multidrug resistance (3 to 5 antibiotics) among all the tested uropathogens.ConclusionsThe obtained results revealed that all the Lactobacillus isolates displayed antimicrobial activity against 6 out of 7 antibiotic-resistant uropathogens, indicating that these bacteria could represent a good ecological plan for the control and prevention of UTI.

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Bioactivity and Control Efficacy of the Novel Antibiotic Tetramycin against Various Kiwifruit Diseases.

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Bioactivity and Control Efficacy of the Novel Antibiotic Tetramycin against Various Kiwifruit Diseases.

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