Dataset Information

The Case for GNMT as a Biomarker and a Therapeutic Target in Pancreatic Cancer.

ABSTRACT: The high mortality rate for pancreatic cancer (PC) is due to the lack of specific symptoms at early tumor stages and a high biological aggressiveness. Reliable biomarkers and new therapeutic targets would help to improve outlook in PC. In this study, we analyzed the expression of GNMT in a panel of pancreatic cancer cell lines and in early-stage paired patient tissue samples (normal and diseased) by quantitative reverse transcription-PCR (qRT-PCR). We also investigated the effect of 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranoside (PGG) as a therapeutic agent for PC. We find that GNMT is markedly downregulated (p < 0.05), in a majority of PC cell lines. Similar results are observed in early-stage patient tissue samples, where GNMT expression can be reduced by a 100-fold or more. We also show that PGG is a strong inhibitor of PC cell proliferation, with an IC50 value of 12 ng/mL, and PGG upregulates GNMT expression in a dose-dependent manner. In conclusion, our data show that GNMT has promise as a biomarker and as a therapeutic target for PC.

SUBMITTER: Heinzman Z

PROVIDER: S-EPMC7998508 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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The Case for GNMT as a Biomarker and a Therapeutic Target in Pancreatic Cancer.

Heinzman Zachary Z Schmidt Connor C Sliwinski Marek K MK Goonesekere Nalin C W NCW

Pharmaceuticals (Basel, Switzerland) 20210303 3

The high mortality rate for pancreatic cancer (PC) is due to the lack of specific symptoms at early tumor stages and a high biological aggressiveness. Reliable biomarkers and new therapeutic targets would help to improve outlook in PC. In this study, we analyzed the expression of GNMT in a panel of pancreatic cancer cell lines and in early-stage paired patient tissue samples (normal and diseased) by quantitative reverse transcription-PCR (qRT-PCR). We also investigated the effect of 1,2,3,4,6-pe ...[more]

PMID: 33802396

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Dataset Information

The Case for GNMT as a Biomarker and a Therapeutic Target in Pancreatic Cancer.

Publications

The Case for GNMT as a Biomarker and a Therapeutic Target in Pancreatic Cancer.

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