Counter-Current Fractionation-Assisted Bioassay-Guided Separation of Active Compound from Blueberry and the Interaction between the Active Compound and α-Glucosidase.

ABSTRACT: An efficient strategy for the selection of active compounds from blueberry based on counter-current fractionation and bioassay-guided separation was established in this study. Blueberry extract showed potential α-glucosidase inhibitory activity. After extraction by different solvents, the active components were enriched in water. The water extract was divided into six fractions via high-speed counter-current chromatography to further track the active components. Results indicated that the α-glucosidase inhibition rate of F4 was remarkable higher than the others. Cyanidin-3-glucoside (C3G) with a purity of 94.16% was successfully separated from F4 through column chromatography, and its structure was identified by ultraviolet spectral, Fourier-transformed infrared spectroscopy, high-performa

SUBMITTER: Xue H 

PROVIDER: S-EPMC7998573 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature