Multiplex Real-Time PCR Assay for Six Major Carbapenemase Genes.

ABSTRACT: The global dissemination of carbapenemase-producing Enterobacteriaceae (CPE) is a major concern in public health. Due to the existence of the diversity of carbapenemases, development of an easily available, cost-effective multiplex detection assay for CPE is required worldwide. Using clinically available and reliable equipment, COBAS^® z480 (Roche Diagnostics K.K., Tokyo, Japan), we developed a multiplex real-time PCR assay for the detection of two combinations of carbapenemases; first, bla_NDM, bla_KPC, and bla_IMP (Set 1), and second, bla_GES, bla_OXA-48, and bla_VIM (Set 2). We constructed standard curves for each carbapenemase gene using serial dilutions of DNA standar

SUBMITTER: Yoshioka N 

PROVIDER: S-EPMC7999841 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature