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Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves.


ABSTRACT: Within microfluidic technologies, the centrifugal microfluidic "Lab-on-a-Disc" (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize 'wet bench' laboratory protocols. We present the combination of 'event-triggered dissolvable film valves' with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL-1 and limit of quantitation (LoQ) of 135.87 ng mL-1.

SUBMITTER: Henderson BD 

PROVIDER: S-EPMC8000095 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves.

Henderson Brian D BD   Kinahan David J DJ   Rio Jeanne J   Mishra Rohit R   King Damien D   Torres-Delgado Sarai M SM   Mager Dario D   Korvink Jan G JG   Ducrée Jens J  

Biosensors 20210306 3


Within microfluidic technologies, the centrifugal microfluidic "Lab-on-a-Disc" (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize 'wet bench' laboratory protocols. We present the combination of 'event-triggered dissolvable film valves' with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagent  ...[more]

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