High-Throughput Cell Trapping in the Dentate Spiral Microfluidic Channel.

ABSTRACT: Cell trapping is a very useful technique in a variety of cell-based assays and cellular research fields. It requires a high-throughput, high-efficiency operation to isolate cells of interest and immobilize the captured cells at specific positions. In this study, a dentate spiral microfluidic structure is proposed for cell trapping. The structure consists of a main spiral channel connecting an inlet and an out and a large number of dentate traps on the side of the channel. The density of the traps is high. When a cell comes across an empty trap, the cell suddenly makes a turn and enters the trap. Once the trap captures enough cells, the trap becomes closed and the following cells pass by the trap. The microfluidic structure is optimized based on the investigation of the influence over the f

SUBMITTER: Lu J 

PROVIDER: S-EPMC8000121 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature