Antenna Protein Clustering In Vitro Unveiled by Fluorescence Correlation Spectroscopy.

ABSTRACT: Antenna protein aggregation is one of the principal mechanisms considered effective in protecting phototrophs against high light damage. Commonly, it is induced, in vitro, by decreasing detergent concentration and pH of a solution of purified antennas; the resulting reduction in fluorescence emission is considered to be representative of non-photochemical quenching in vivo. However, little is known about the actual size and organization of antenna particles formed by this means, and hence the physiological relevance of this experimental approach is questionable. Here, a quasi-single molecule method, fluorescence correlation spectroscopy (FCS), was applied during in vitro quenching of LHCII trimers from higher plants for a parallel estimation of particle size, fluorescence, and antenna clus

SUBMITTER: Crepin A 

PROVIDER: S-EPMC8000295 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature