Analysis of Morphokinetic Parameters of Feline Embryos Using a Time-Lapse System.

ABSTRACT: The aim of this study was to analyze the morphokinetic parameters of feline embryos using a time lapse system. Oocytes matured in vitro were fertilized (IVF) and in vitro cultured in a time lapse-system (Primo Vision®, Gothenburg, Sweden). The first cell division of embryos occurred between 17 h post insemination (hpi) and 38 hpi, with the highest proportion of embryos (46%) cleaving between 21 and 24 hpi. The timing of the first cleavage significantly affected further embryo development, with the highest development occurring in embryos that cleaved at 21-22 hpi. Embryos that cleaved very early (17-18 hpi) developed poorly to the blastocyst stage (2%) and none of the embryos that cleaved later than 27 hpi were able to reach the blastocyst stage. Morphological defects were observed in 48%

SUBMITTER: Kochan J 

PROVIDER: S-EPMC8000546 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature