A Novel High Throughput, Parallel Infection Assay for Determining the Replication Capacities of 346 Primary HIV-1 Isolates of the Zurich Primary HIV-1 Infection Study in Primary Cells.

ABSTRACT: HIV-1 replication capacity is an important characteristic to understand the replication competence of single variants or virus populations. It can further aid in the understanding of HIV-1 pathogenicity, disease progression, and drug resistance mutations. To effectively study RC, many assays have been established. However, there is still demand for a high throughput replication capacity assay using primary cells which is robust and reproducible. In this study, we established such an assay and validated it using 346 primary HIV-1 isolates from patients enrolled in the Zurich Primary HIV Infection study (ZPHI) and two control viruses, HIV-1 JR-CSF_WT and HIV-1 JR-CSF_{K65R_M184V}. Replication capacity was determined by measuring the viral growth on PBMCs over 10 days by lon

SUBMITTER: Rindler AE 

PROVIDER: S-EPMC8000554 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature