An Open-Source Add-On EVOM^® Device for Real-Time Transepithelial/Endothelial Electrical Resistance Measurements in Multiple Transwell Samples.

ABSTRACT: This study provides design of a low-cost and open source add-on device that enhances the functionality of the popular EVOM^® instrument for transepithelial/endothelial electrical resistance (TEER) measurement. The original EVOM^® instrument is designed for measuring TEER in transwell samples manually using a pair of Ag/AgCl electrodes. The inconsistency in electrode placement, temperature variation, and a typically large (12-24 h) time interval between measurements result in large data variabilities. Thus, to solve the current limitation of the EVOM^® instrument, we built an add-on device using a custom designed electronic board and a 3D printed electrode holder that allowed automated TEER measurements in multiple transwell samples. To demonstrate the function

SUBMITTER: Raut B 

PROVIDER: S-EPMC8000980 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature