Descriptors of Secondary Active Transporter Function and How They Relate to Partial Reactions in the Transport Cycle.

ABSTRACT: Plasmalemmal solute carriers (SLCs) gauge and control solute abundance across cellular membranes. By virtue of this action, they play an important role in numerous physiological processes. Mutations in genes encoding the SLCs alter amino acid sequence that often leads to impaired protein function and onset of monogenic disorders. To understand how these altered proteins cause disease, it is necessary to undertake relevant functional assays. These experiments reveal descriptors of SLC function such as the maximal transport velocity (Vmax), the Michaelis constant for solute uptake (KM), potencies for inhibition of transporter function (IC50/EC50), and many more. In several instances, the mutated versions of different SLC transporters differ from their wild-type counterparts in the value of t

SUBMITTER: Schicker K 

PROVIDER: S-EPMC8001282 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature