Coupling Microplate-Based Antibacterial Assay with Liquid Chromatography for High-Resolution Growth Inhibition Profiling of Crude Extracts: Validation and Proof-of-Concept Study with Staphylococcus aureus.

ABSTRACT: With the identification of novel antibiotics from nature being pivotal in the fight against human pathogenic bacteria, there is an urgent need for effective methodologies for expedited screening of crude extracts. Here we report the development and validation of a simple and dye-free antimicrobial assay in 96-well microplate format, for both determination of IC₅₀ values and high-resolution inhibition profiling to allow pin-pointing of bioactive constituents directly from crude extracts. While commonly used antimicrobial assays visualize cell viability using dyes, the developed and validated assay conveniently uses OD₆₀₀ measurements directly on the fermentation broth. The assay was validated with an investigation of the inhibitory activity of DMSO against Staphyloc

SUBMITTER: Ardalani H 

PROVIDER: S-EPMC8001363 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature