Identification of Ku70 Domain-Specific Interactors Using BioID2.

ABSTRACT: Since its inception, proximity-dependent biotin identification (BioID), an in vivo biochemical screening method to identify proximal protein interactors, has seen extensive developments. Improvements and variants of the original BioID technique are being reported regularly, each expanding upon the existing potential of the original technique. While this is advancing our capabilities to study protein interactions under different contexts, we have yet to explore the full potential of the existing BioID variants already at our disposal. Here, we used BioID2 in an innovative manner to identify and map domain-specific protein interactions for the human Ku70 protein. Four HEK293 cell lines were created, each stably expressing various BioID2-tagged Ku70 segments designed to collectively identify

SUBMITTER: Abbasi S 

PROVIDER: S-EPMC8001828 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature