Processing Matters in Nutrient-Matched Laboratory Diets for Mice-Microbiome.

ABSTRACT: The composition of the microbiome is subject to the host's diet. In commercial laboratory mouse diets, different physical forms of the same diets are available, containing-according to their labels-identical ingredients and nutrient compositions. However, variations in nutrient composition and starch gelatinization due to production processes and their impact on digestibility have been described. In this study, a total of 48 C57BL/J6 mice were assigned to two equal groups and were fed diets (produced with different processes-extruded vs. pelleted) for eight weeks in two biological replicates. At the end of the experiment, samples were collected from five different gastrointestinal regions, including the stomach, small intestine, cecum, large intestine, and an extracorporeal region (feces),

SUBMITTER: Wenderlein J 

PROVIDER: S-EPMC8002992 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature