An Amplicon-Based Approach for the Whole-Genome Sequencing of Human Metapneumovirus.

ABSTRACT: Human metapneumovirus (HMPV) is an important cause of upper and lower respiratory tract disease in individuals of all ages. It is estimated that most individuals will be infected by HMPV by the age of five years old. Despite this burden of disease, there remain caveats in our knowledge of global genetic diversity due to a lack of HMPV sequencing, particularly at the whole-genome scale. The purpose of this study was to create a simple and robust approach for HMPV whole-genome sequencing to be used for genomic epidemiological studies. To design our assay, all available HMPV full-length genome sequences were downloaded from the National Center for Biotechnology Information (NCBI) GenBank database and used to design four primer sets to amplify long, overlapping amplicons spanning the viral gen

SUBMITTER: Tulloch RL 

PROVIDER: S-EPMC8003040 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature