Development of genic KASP SNP markers from RNA-Seq data for map-based cloning and marker-assisted selection in maize.

ABSTRACT:

Background

Maize is one of the most important field crops in the world. Most of the key agronomic traits, including yield traits and plant architecture traits, are quantitative. Fine mapping of genes/ quantitative trait loci (QTL) influencing a key trait is essential for marker-assisted selection (MAS) in maize breeding. However, the SNP markers with high density and high polymorphism are lacking, especially kompetitive allele specific PCR (KASP) SNP markers that can be used for automatic genotyping. To date, a large volume of sequencing data has been produced by the next generation sequencing technology, which provides a good pool of SNP loci for development of SNP markers. In this study, we carried out a multi-step screening method to identify kompetitive allele specific PCR (KAS

SUBMITTER: Chen Z 

PROVIDER: S-EPMC8004444 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature