Molecular characteristics of Brucella melitensis isolates from humans in Qinghai Province, China.

ABSTRACT:

Background

The prevalence of human brucellosis in Qinghai Province of China has been increasing rapidly, with confirmed cases distributed across 31 counties. However, the epidemiology of brucellosis transmission has not been fully elucidated. To characterize the infecting strains isolated from humans, multiple-locus variable-number tandem repeats analysis (MLVA) and whole-genome single-nucleotide polymorphism (SNP)-based approaches were employed.

Methods

Strains were isolated from two males blood cultures that were confirmed Brucella melitensis positive following biotyping and MLVA. Genomic DNA was extracted from these two strains, and whole-genome sequencing was performed. Next, SNP-based phylogenetic analysis was performed to compare the two strains to 94 B. melitensis str

SUBMITTER: Zhao ZJ 

PROVIDER: S-EPMC8004457 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature