Growth cone repulsion to Netrin-1 depends on lipid raft microdomains enriched in UNC5 receptors.

ABSTRACT: During brain development, Uncoordinated locomotion 5 (UNC5) receptors control axonal extension through their sensing of the guidance molecule Netrin-1. The correct positioning of receptors into cholesterol-enriched membrane raft microdomains is crucial for the efficient transduction of the recognized signals. However, whether such microdomains are required for the appropriate axonal guidance mediated by UNC5 receptors remains unknown. Here, we combine the use of confocal microscopy, live-cell FRAP analysis and single-particle tracking PALM to characterize the distribution of UNC5 receptors into raft microdomains, revealing differences in their membrane mobility properties. Using pharmacological and genetic approaches in primary neuronal cultures and brain cerebellar explants we further dem

SUBMITTER: Hernaiz-Llorens M 

PROVIDER: S-EPMC8004515 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature