Cell Surface Profiling of Retinal Muller Glial Cells Reveals Association to Immune Pathways after LPS Stimulation.

ABSTRACT: Retinal Müller glial cells (RMG) are involved in virtually every retinal disease; however, the role of these glial cells in neuroinflammation is still poorly understood. Since cell surface proteins play a decisive role in immune system signaling pathways, this study aimed at characterizing the changes of the cell surface proteome of RMG after incubation with prototype immune system stimulant lipopolysaccharide (LPS). While mass spectrometric analysis of the human Müller glia cell line MIO-M1 revealed 507 cell surface proteins in total, with 18 proteins significantly more abundant after stimulation (ratio ≥ 2), the surfaceome of primary RMG comprised 1425 proteins, among them 79 proteins with significantly higher abundance in the stimulated state. Pathway analysis revealed notable associati

SUBMITTER: Lorenz L 

PROVIDER: S-EPMC8004686 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature