MiRNA Expression Characterizes Histological Subtypes and Metastasis in Penile Squamous Cell Carcinoma.

ABSTRACT: Although microRNAs are described as promising biomarkers in many tumor types, little is known about their role in PSCC. Thus, we attempted to identify miRNAs involved in tumor development and metastasis in distinct histological subtypes considering the impact of HPV infection. In a first step, microarray analyses were performed on RNA from formalin-fixed, paraffin-embedded tumor (22), and normal (8) tissue samples. Microarray data were validated for selected miRNAs by qRT-PCR on an enlarged cohort, including 27 tumor and 18 normal tissues. We found 876 significantly differentially expressed miRNAs (p ≤ 0.01) between HPV-positive and HPV-negative tumor samples by microarray analysis. Although no significant differences were detected between normal and tumor tissue in the whole cohort

SUBMITTER: Ayoubian H 

PROVIDER: S-EPMC8004785 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature