Stoichiometric Thiol Redox Proteomics for Quantifying Cellular Responses to Perturbations.

ABSTRACT: Post-translational modifications regulate the structure and function of proteins that can result in changes to the activity of different pathways. These include modifications altering the redox state of thiol groups on protein cysteine residues, which are sensitive to oxidative environments. While mass spectrometry has advanced the identification of protein thiol modifications and expanded our knowledge of redox-sensitive pathways, the quantitative aspect of this technique is critical for the field of redox proteomics. In this review, we describe how mass spectrometry-based redox proteomics has enabled researchers to accurately quantify the stoichiometry of reversible oxidative modifications on specific cysteine residues of proteins. We will describe advancements in the methodology that al

SUBMITTER: Day NJ 

PROVIDER: S-EPMC8004825 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature