Fully synthetic matrices for in vitro culture of primary human intestinal enteroids and endometrial organoids.

ABSTRACT: Epithelial organoids derived from human donor tissues are important tools in fields ranging from regenerative medicine to drug discovery. Organoid culture requires expansion of stem/progenitor cells in Matrigel, a tumor-derived extracellular matrix (ECM). An alternative completely synthetic ECM could improve reproducibility, clarify mechanistic phenomena, and enable human implantation of organoids. We designed synthetic ECMs with tunable biomolecular and biophysical properties to identify gel compositions supporting human tissue-derived stem/progenitor epithelial cells as enteroids and organoids starting with single cells rather than tissue fragments. The synthetic ECMs consist of 8-arm PEG-macromers modified with ECM-binding peptides and different combinations of integrin-binding peptides

SUBMITTER: Hernandez-Gordillo V 

PROVIDER: S-EPMC8005336 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature