Improved Yield of Recombinant Protein via Flagella Regulator Deletion in Escherichia coli.

ABSTRACT: Protein production requires a significant amount of intracellular energy. Eliminating the flagella has been proposed to help Escherichia coli improve protein production by reducing energy consumption. In this study, the gene encoding a subunit of FlhC, a master regulator of flagella assembly, was deleted to reduce the expression of flagella-related genes. FlhC knockout in the ptsG-deleted strain triggered significant growth retardation with increased ATP levels and a higher NADPH/NADP⁺ ratio. Metabolic flux analysis using a ¹³C-labeled carbon substrate showed increased fluxes toward the pentose phosphate and tricarboxylic acid cycle pathways in the flhC- and ptsG-deleted strains. Introduction of a high copy number plasmid or overexpression

SUBMITTER: Han JH 

PROVIDER: S-EPMC8005581 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature