Profiling Tissue and Biofluid miR-155-5p, miR-155^*, and miR-146a-5p Expression in Graft vs. Host Disease.

ABSTRACT: Introduction: Acute graft vs. host disease (aGvHD) is a frequent complication following allogeneic haematopoeitic transplantation (HSCT). Despite recent advances, there are no universally accepted biomarkers to determine development of aGvHD. MicroRNAs miR-146a and miR-155 have been previously associated with aGvHD and show promise as clinically translatable biomarkers. In this study, we performed comprehensive expression profiling of miR-146a, miR-155, and miR-155^* expression in aGvHD target tissue and biofluids and relate expression to post-HSCT outcomes. Materials and Methods: MicroRNA expression was assessed by qRT-PCR in gastrointestinal (n = 31) and skin (n = 31) biopsies as well as serum (exploratory cohort n = 34, verification cohort

SUBMITTER: Crossland RE 

PROVIDER: S-EPMC8005601 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature