Membrane Lipid Requirements of the Lysine Transporter Lyp1 from Saccharomyces cerevisiae.

ABSTRACT: Membrane lipids act as solvents and functional cofactors for integral membrane proteins. The yeast plasma membrane is unusual in that it may have a high lipid order, which coincides with low passive permeability for small molecules and a slow lateral diffusion of proteins. Yet, membrane proteins whose functions require altered conformation must have flexibility within membranes. We have determined the molecular composition of yeast plasma membrane lipids located within a defined diameter of model proteins, including the APC-superfamily lysine transporter Lyp1. We now use the composition of lipids that naturally surround Lyp1 to guide testing of lipids that support the normal functioning of the transporter, when reconstituted in vesicles of defined lipid composition. We find that phosphatid

SUBMITTER: van 't Klooster JS 

PROVIDER: S-EPMC8005870 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature