Chloroquine, an Anti-Malaria Drug as Effective Prevention for Hantavirus Infections.

ABSTRACT: We investigated whether chloroquine can prevent hantavirus infection and disease in vitro and in vivo, using the Hantaan virus newborn C57BL/6 mice model and the Syrian hamster model for Andes virus. In vitro antiviral experiments were performed using Vero E6 cells, and Old World and New World hantavirus species. Hantavirus RNA was detected using quantitative RT-PCR. For all hantavirus species tested, results indicate that the IC₅₀ of chloroquine (mean 10.2 ± 1.43 μM) is significantly lower than the CC₅₀ (mean 260 ± 2.52 μM) yielding an overall selectivity index of 25.5. We also investigated the potential of chloroquine to prevent death in newborn mice after Hantaan virus infection and its antiviral effect in the hantavirus Syrian hamster model. F

SUBMITTER: Vergote V 

PROVIDER: S-EPMC8006394 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature