Mass spectrometry reveals potential of β-lactams as SARS-CoV-2 M^pro inhibitors.

ABSTRACT: The main viral protease (Mpro) of SARS-CoV-2 is a nucleophilic cysteine hydrolase and a current target for anti-viral chemotherapy. We describe a high-throughput solid phase extraction coupled to mass spectrometry Mpro assay. The results reveal some β-lactams, including penicillin esters, are active site reacting Mpro inhibitors, thus highlighting the potential of acylating agents for Mpro inhibition.

SUBMITTER: Malla TR 

PROVIDER: S-EPMC8006714 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature