Microfluidics-Coupled Radioluminescence Microscopy for In Vitro Radiotracer Kinetic Studies.

ABSTRACT: Integrated bioassay systems that combine microfluidics and radiation detectors can deliver medical radiopharmaceuticals to live cells with precise timing, while minimizing radiation dose and sample volume. However, the spatial resolution of many radiation imaging systems is limited to bulk cell populations. Here, we demonstrate microfluidics-coupled radioluminescence microscopy (μF-RLM), a new integrated system that can image radiotracer uptake in live adherent cells growing inside microincubators with spatial resolution better than 30 μm. Our method enables on-chip radionuclide imaging by incorporating an inorganic scintillator plate (CdWO₄) into a microfluidic chip. We apply this approach to investigate the factors that influence the dynamic uptake of [¹⁸F]fluorodeo

SUBMITTER: Kim TJ 

PROVIDER: S-EPMC8006742 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature